中文版

Gut

粪便代谢物谱发现中链脂肪酸可作为判断炎症性肠病疾病活动度的指标
 

背景:肠道菌群与IBD的发生和发展有关。细菌组成和结构的变化可能引起肠道菌群代谢能力的改变。
 

目的：比较CD、UC、隐窝炎病人与健康人肠道菌群代谢活性的差异，并判断这些差异与IBD的发病机制是否有关。
 

方法：收集40例健康人、83例CD患者、68例UC患者、13例隐窝炎患者的粪便样本。分别用HBI、UCDAI、PDAI评分评估CD、UC、隐窝炎患者的疾病活动度。使用气相色谱-质谱技术分析粪便代谢物谱。
 

结果：健康组鉴定的代谢产物（54种）明显高于CD组（44种，p<0.001）、UC组（47种，p=0.042）及隐窝炎组（36种，p=0.036）。多元判别分析发现健康组、CD组、UC组的代谢物谱具有高灵敏性和特异性。与健康组相比，CD、UC及隐窝炎组的中链脂肪酸（戊酸、已酸、庚酸、辛酸、壬酸）和一些蛋白发酵产物明显降低。已酸含量与CD疾病活动度呈负相关（相关系数= −0.157,p=0.046），而苯乙烯含量与UC疾病活动度呈明显正相关（相关系数=0.338,p=0.001）。
 

结论：粪便代谢物谱发现中链脂肪酸是判断IBD疾病活动度变化的重要代谢指标。
 

摘自：Gut 2014;0:1-12. doi:10.1136/gutjnl-2013-306423

（译：张发明）

南京医科大学第二附属医院肠病中心

原文链接: http://gut.bmj.com/content/early/2014/05/08/gutjnl-2013-306423.abstract?sid=1c41b133-755b-45e5-9ab7-013b0c75960c

英文版

Gut

Faecalmetabolite profiling identifies medium-chain fatty acids as discriminatingcompounds in IBD

Background:Bacteria play arole in the onset and perpetuation of intestinal inflammation in IBD.Compositional alterations may also change the metabolic capacities of the gutbacteria.
 

Objective:To examine themetabolic activity of the microbiota of patients with Crohn’s disease (CD), UCor pouchitis compared with healthy controls (HC) and determine whether eventualdifferences might be related to the pathogenesis of the disease.
 

Methods:Faecal sampleswere obtained from 40 HC, 83 patients with CD, 68 with UC and 13 withpouchitis. Disease activity was assessed in CD using the Harvey–Bradshaw Index,in UC using the UC Disease Activity Index and in pouchitis using the PouchitisDisease Activity Index. Metabolite profiles were analysed using gaschromatography–mass spectrometry.
 

Results:The number ofmetabolites identified in HC (54) was significantly higher than in patientswith CD (44, p<0.001), UC (47, p=0.042) and pouchitis (43, p=0.036). Multivariatediscriminant analysis predicted HC, CD, UC and pouchitis group membership withhigh sensitivity and specificity. The levels of medium-chain fatty acids(MCFAs: pentanoate, hexanoate, heptanoate, octanoate and nonanoate), and ofsome protein fermentation metabolites, were significantly decreased in patientswith CD, UC and pouchitis. Hexanoate levels were inversely correlated to diseaseactivity in CD (correlation coefficient=−0.157, p=0.046), whereas a significantpositive correlation was found between styrene levels and disease activity inUC (correlation coefficient=0.338, p=0.001).
 

Conclusions:Faecal metabolicprofiling in patients with IBD relative to healthy controls identified MCFAs asimportant metabolic biomarkers of disease-related changes.

From：Gut2014;0:1–12. doi:10.1136/gutjnl-2013-306423

【Originalconnection】 http://gut.bmj.com/content/early/2014/05/08/gutjnl-2013-306423.abstract?sid=1c41b133-755b-45e5-9ab7-013b0c75960c